Mouse with tumor ulceration of appr

Mouse with tumor ulceration of approximately 25% or greater on the surface of the tumor would be euthanized.General Animal Welfare SurveillanceSevere dehydration, hypothermia, abnormal/labored respiration, lethargy, obvious pain, diarrhea, skin lesions, neurological symptoms, impaired mobility(not able to eat or drink)due to significant ascites and enlarged abdomen, astasia, continuous prone or lateral position, signs of muscular atrophy, paralytic gait, clonic convulsions, tonic convulsions, persistent bleeding from body orifice.6.3 Terminal Sample CollectionNo sample were collected in this study.To compare tumor volumes of different groups at a pre-specified day, we first used Bartlett's test to check the assumption of homogeneity of variance across all groups.When the p-value of Bartlett's test was =0.05, we ran one-way ANOVA to test overall equality of means across all groups.If the p-value of the one-way ANOVA was 0.05, we further performed post hoc testing by running Tukey's HSD(honest significant difference)tests for all pairwise comparisons, and Dunnett's tests for comparing each treatment group with the vehicle group.When the p-value of Bartlett's test was 0.05, we ran Kruskal-Wallis test to test overall equality of medians among all groups.If the p- value the Kruskal-Wallis test was 0.05, we further performed post hoc testing by running Conover's non-parametric test for all pairwise comparisons or for comparing each treatment group with the vehicle group, both with single-step p-value adjustment.In addition, we performed pairwise comparisons without multiple comparison correction and reported nominal/uncorrected p-values directly from Welch's t-test or Mann-Whitney U test.Specifically, we first used Bartlett's test to check the assumption of homogeneity of variance for a pair of groups.When the p-value of Bartlett's test was 0.05, we run Welch's t-test, otherwise we ran Mann-Whitney U test, to obtain nominal p-values.All statistical analyses had been done in R-a language and environment for statistical computing and graphics(version 3.3.1).All tests were two-sided unless otherwise specified, and p-values of