3. The basic principles and classif

3. The basic principles and classification of chemiluminescence immunoassay analyzers Chemiluminescence immunoassay products have been commercialized and industrialized internationally, and can basically be divided into chemiluminescence immunoassay and electrochemiluminescence immunoassay. Chemiluminescence is a chemical reaction (usually an oxidation reaction) that produces substances whose electronic energy levels are in an excited state. The latter releases energy through electronic transitions to generate photons, leading to luminescence. The analytical method established based on the relationship between the chemiluminescence intensity and the content of the analyte is called chemiluminescence analysis. It has the characteristics of high sensitivity, wide linear range, simple instrument and convenient operation. It has been widely used in environmental, clinical, food and industrial analysis. According to the type of reaction, chemiluminescence can be divided into enzymatic chemiluminescence and non-chemiluminescence. Among them, enzymatic chemiluminescence mainly includes horseradish peroxidase (HRP) system and alkaline-encoding oxidase (AP) system. Non-enzymatic chemiluminescence mainly includes the acridine system. According to the duration of light emission, it can be divided into flash and glow type. Among them, the flash emission time is short, in seconds, such as the acridine system. Generally, the in-situ sampling Cinmsituinjector and the heart-to-heart integration method are used for measurement, that is, an injector is installed at the detector to ensure that the two processes of adding luminescent agent and detecting are carried out simultaneously. At the same time, the area of ​​the entire luminescence signal peak is the luminescence intensity: the glow-type luminescence time is several minutes to several tens of minutes, such as horseradish peroxidase luminol system and alkaline phosphatase AMPPD system. Signal detection does not require in-situ sampling. Generally, the rate method is used to measure the luminous intensity per unit time (flat area) at any point in an area where the luminous signal is relatively stable.