2.6 General Procedure for Bilirubin DetectionThe typical procedure for the detection of bilirubin is as follows. 3 mL of the CuNCswas half quenched with 20 µL of 10 mM Fe3+ (66 µM) at pH 11.4. Then, equivalents ofbilirubin were added stepwise. Fluorescence measurements were taken immediately after theaddition of bilirubin. Similar procedure was followed to investigate the interference of probetowards bilirubin over other coexisting biomolecules (creatine, galactose, glucose, fructose, urea, uric acid, glutathione (GSH), cholesterol and haemoglobin). Control experiments werecarried out with water to nullify the effect caused by the dilution. All the experiments weredone in triplicate and batch to batch reproducibility was confirmed.