Open-tip exchange times between lum

Open-tip exchange times between lumen of the barrel were determined by perfusion of low- and high-electrolyte solutions and were consistently less than 1ms. For analysis of current density, the pipette capacitance (13 pF) was subtracted from the cell capacitance and the peak current amplitude was normalized to the capacitance of the cell.All data was compared with the use of a Student’s paired t test, with Welch’s correction when variances were significantly different, and plotted with the use of GraphPad Software (San Diego, CA).