Hofstetter et al. (2002) based on the phylogenetic analysis of ITS-LSU-SU three genes, the results showed that Calocybe can be used as a monophyletic group, which was supported by several studies (Moncalvo et al., 2002, Hofstetter et al., 2014 Year). Subsequently, Bellanger et al. (2015) confirmed the inclusion of some Rugomyces species in vesicular bacteria by multigene phylogenetic analysis of ITS-RPB2.