To determine the genotype of CYP2C1

To determine the genotype of CYP2C19*2 and CYP2C19*3, two sets of PCR primers were designed in this study. The expected PCR product size of CYP2C19*2 and CYP2C19*3 is 466 bp and 500bp, respectively. Gel electrophoresis (Fig. 1A) confirmed that the size of the PCR product corresponded to the expected size (displays a section of the PCR results, n=30). Fig. 1B shows the corresponding sequencing results, show that there were no overlapping peaks on the sequenced peak map, except for in the mutation sites of the samples with heterozygous mutations. To confirm the results, the PCR amplifications were performed twice for each sample, and direct sequenced. These results suggested that the PCR primes had good specificity, and the sequencing results can be used as a gold standard for evaluate the accuracy of LAMP detection.