In addition, compared to thegroup undersham surgery,weobservedthatTwist1wassignificantlyupregulatedinCCI rats in a time‐dependent manner (Figure 5A).Compared with the CCI control group, after miR‐134‐5pwas overexpressed in CCI rats, it was found that Twist1mRNA and protein levels were significantly inhibited byLV‐miR‐134‐5p(Figure5B‐D).Theseresultsdemonstratedthat Twist1 was repressed by miR‐134‐5p overexpressioninvivo.