The contextual fear test was conducted as described previously [18]. In brief, mice were placed in a contextual chamber (32 × 26 × 26 cm operant chambers) for an acclimation period (180s). Then, mice were treated with a pure tone (15 s, 1 kHz, 90 dB) and co-terminated with a foot shock (1 s, 0.8 mA) for three times. Afterwards, mice were kept in the chamber for another 2 min. 24 hours after fear conditioning training, mice were replaced in the contextual chamber for 5 min without tone or foot shock exposure, and the freezing time of mice was recorded.
The contextual fear test was conducted as described previously [18]. In brief, mice were placed in a contextual chamber (32 × 26 × 26 cm operant chambers) for an acclimation period (180s). Then, mice were treated with a pure tone (15 s, 1 kHz, 90 dB) and co-terminated with a foot shock (1 s, 0.8 mA) for three times. Afterwards, mice were kept in the chamber for another 2 min. 24 hours after fear conditioning training, mice were replaced in the contextual chamber for 5 min without tone or foot shock exposure, and the freezing time of mice was recorded.
正在翻译中..